DNA

Part:BBa_M45603:Design

Designed by: Anna Doloman   Group: UtahState_BE5930_-_S14   (2015-03-27)


VDRE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Should be incorporated into the promoter, which needs to be regulated by binding of VDR protein to this sequence - VDRE.

Source

Binding site of the VDR (vitamin D receptor) protein, that has already incorporated a molecule of vitamin D. VDRE element is located in the promoter of osteocalcin gene of Homo sapiens and regulates transcription based on the amount of vitamin D in the cell.

References

Mottershead DG, Polly P, Lyons RJ, Sutherland RL & Watts CK (1996) High activity, soluble, bacterially expressed human vitamin D receptor and its ligand binding domain. J Cell Biochem 61: 325-337.